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KMID : 1161420190220020196
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Journal of Medicinal Food
2019 Volume.22 No. 2 p.196 ~ p.201
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Synergistic Effect of Two Major Components of Malva verticillata in the Recovery of Alloxan-Damaged Pancreatic Islet Cells in Zebrafish
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Ko Jung-Hwan
Rodriguez Isabel
Joo Sun-Woo
Kim Hyoung-Geun
Lee Yeong-Geun
Kang Tong-Ho
Baek Nam-In
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Abstract
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Malva verticillata (Chinese mallow) has long been used in traditional medicines and herbal teas in Asia. The n-BuOH fraction (Fr) from M. verticillata promoted significant recovery of alloxan-damaged (AXD) pancreatic islets (PIs) in zebrafish (ZF). Two major components were isolated from M. verticillata through repeated-column chromatography. Based on several spectroscopic methods, including nuclear magnetic resonance (NMR), infrared spectroscopy (IR), and fast atom bombardment-mass spectrometry (FAB-MS), the chemical structures of compounds 1 and 2 were determined. In addition, the quantity of both compounds in the n-BuOH Fr was investigated through high-performance liquid chromatography (HPLC) and the quantities of compounds 1 and 2 in the n-BuOH Fr were determined to be 5.58%?±?0.16% and 2.85%?±?0.13%, respectively. The n-BuOH Fr, compounds 1 and 2, and the mixture of compounds 1 and 2 (MX, 1 and 2, the ratio of both compounds in n-BuOH Fr, 1.96:1) were evaluated for their ability to recover AXD PIs and for their KATP channel-blocking mechanism using diazoxide in ZF. The n-BuOH Fr (10?μg/mL) and compounds 1 and MX (1?μg/mL) exhibited a recovery effect on AXD PIs. The n-BuOH Fr (10?μg/mL) and MX (1?μg/mL) were also confirmed to be useful KATP channel activators. A synergistic effect of MX in the recovery of AXD PIs was first confirmed in ZF, and it was discovered that 2 acted as an insulin sensitivity activator that increased the activity of compound 1.
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KEYWORD
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3,5,6,9-tetrahydroxy-7-megastigmene, antidiabetes, L-tryptophan, Malva verticillata, synergistic effect, zebrafish
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